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. 2006 Dec;13(12):1363-6.
doi: 10.1128/CVI.00239-06. Epub 2006 Oct 11.

Diagnosis of Paracoccidioidomycosis by detection of antigen and antibody in bronchoalveolar lavage fluids

Silvia Helena Marques-da-Silva  1 , Arnaldo Lopes Colombo, Maria Heloisa Souza Lima Blotta, Flávio Queiroz-Telles, Alípio Barbosa Balthazar, José Daniel Lopes, Zoilo Pires de Camargo
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Diagnosis of Paracoccidioidomycosis by detection of antigen and antibody in bronchoalveolar lavage fluids

Silvia Helena Marques-da-Silva et al. Clin Vaccine Immunol. 2006 Dec.

Abstract

Paracoccidioidomycosis (PCM) is a systemic infection caused by the fungus Paracoccidioides brasiliensis and is believed to be the leading cause of fungal pulmonary infection. In this study, we used an inhibition enzyme-linked immunosorbent assay to diagnose pulmonary PCM based on the detection of 43-kDa and 70-kDa molecules in bronchoalveolar lavage fluids. The results were compared with results obtained by classical methods for antibody detection.

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References

    1. Blotta, M. H. S. L., R. L. Mamoni, S. J. Oliveira, A. S. Nouér, P. M. O. Papaiordanou, A. Gouveia, and Z. P. de Camargo. 1999. Endemic regions of paracoccidioidomycosis in Brazil: a clinical and epidemiologic study of 584 cases in southeast region. Am. J. Trop. Med. Hyg. 61:390-394. - PubMed
    1. Bradford, M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254. - PubMed
    1. Brummer, E., E. Castañeda, and A. Restrepo. 1993. Paracoccidioidomycosis: an update. Clin. Microbiol. Rev. 2:89-117. - PMC - PubMed
    1. de Camargo, Z. P., C. Unterkircher, S. P. Campoy, and L. R. Travassos. 1988. Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J. Clin. Microbiol. 26:2147-2151. - PMC - PubMed
    1. de Camargo, Z. P., R. Berzaghi, C. C. Amaral, and S. H. Marques-da-Silva. 2003. Simplified method for producing Paracoccidioides brasiliensis exoantigens for use in immunodiffusion tests. Med. Mycol. 41:539-542. - PubMed

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