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. 2006 Oct 14;12(38):6207-11.
doi: 10.3748/wjg.v12.i38.6207.

Interrelationship between chromosome 8 aneuploidy, C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma

Danielle-Queiroz Calcagno  1 , Mariana-Ferreira Leal, Aline-Damaceno Seabra, Andre-Salim Khayat, Elizabeth Suchi Chen, Samia Demachki, Paulo Pimentel Assumpção, Mario Henrique Girão Faria, Silvia Helena Barem Rabenhorst, Márcia Valéria Pitombeira Ferreira, Marília de Arruda Cardoso Smith, Rommel-Rodríguez Burbano
Affiliations

Interrelationship between chromosome 8 aneuploidy, C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma

Danielle-Queiroz Calcagno et al. World J Gastroenterol. .

Abstract

Aim: To investigate chromosome 8 numerical aberrations, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histopathological characteristics of gastric tumors.

Methods: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immunostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed.

Results: All the cases showed chromosome 8 aneuploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level of chromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal-type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm.

Conclusion: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic pathways.

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Figures

Figure 1
Figure 1
Cells submitted to immunohistochemistry and FISH techniques. A: Infiltrating gastric adenocarcinoma of intestinal type shows intense nuclear marcation for C-MYC, × 400; B: Gastric adenocarcinoma of diffuse type nuclear marcation and cytoplasmatic light marcation for C-MYC, × 400; C: Interphase nuclei presenting C-MYC high amplification (red) and chromosome 8 (green); D: Interphase nuclei presenting chromosome 8/C-MYC rearrangement.
See this image and copyright information in PMC

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