Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction

Abstract

The aim of this work was to measure the myeloperoxidase (MPO) concentration in bronchoalveolar lavage (BAL) fluid collected from horses with recurrent airway obstruction (RAO), both in crisis and in remission, as well as from healthy horses. Seven horses with RAO were exposed to moldy hay until the maximum change in pleural pressure was greater than 1.5 kPa. At that point, BAL was performed, and the total cell counts and percentages in the fluid were immediately determined. To measure the MPO concentration in BAL-fluid supernatant, we used a specific enzyme-linked immunosorbent assay with polyclonal antibodies against equine MPO. The tests were repeated on the horses with RAO after they had spent 2 mo on pasture. Six healthy horses serving as controls underwent the same tests. The absolute and relative neutrophil counts and the MPO concentration in the BAL fluid were significantly greater in the horses with an RAO crisis than in the control horses. After 2 mo on pasture, the horses that had been in RAO crisis were clinically normal, and their neutrophil counts and MPO levels in BAL fluid had significantly decreased; during remission their neutrophil counts were not significantly different from those in the healthy horses, but their MPO concentration remained significantly higher. This study showed that determining the MPO concentration in a horse's BAL fluid is technically possible and that during remission from RAO the concentration remains higher than normal. Thus, MPO may be a marker of neutrophil presence and activation in the lower airways.

Figure 1

Relative and absolute numbers of neutrophils (mean ± standard deviation) in the bronchoalveolar lavage (BAL) fluid of 6 healthy horses and 7 horses with recurrent airway obstruction (RAO) in remission after 2 mo on pasture (RAO rem) or in crisis. The asterisks indicate significant differences from the means for the other 2 groups (P < 0.05).

Figure 2

Myeloperoxidase concentration (mean ± standard deviation) in BAL fluid collected from the same groups of horses. The asterisk indicates significant differences from the means for the other 2 groups, and the cross indicates a significant difference from the mean for the healthy horses (P < 0.05)