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. 1990 Oct;60(1-2):29-33.
doi: 10.1016/0378-1097(90)90339-r.

Development of sensitive methods for the detection of mycobacteria by DNA probes

G Rahav  1 , S Sela, H Bercovier
Affiliations

Development of sensitive methods for the detection of mycobacteria by DNA probes

G Rahav et al. FEMS Microbiol Lett. 1990 Oct.

Abstract

A sensitive method allowing the detection of mycobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.

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