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. 2006;2006(4):13901.
doi: 10.1155/MI/2006/13901.

Plasma levels of t-PA and PAI-1 correlate with the formation of experimental post-surgical peritoneal adhesions

Clara Di Filippo  1 , Alessandro Falsetto, Vito De Pascale, Elisabetta Tufariello, Domenico De Lucia, Francesco Rossi, Michele D'Amico, Antonio Cennamo
Affiliations

Plasma levels of t-PA and PAI-1 correlate with the formation of experimental post-surgical peritoneal adhesions

Clara Di Filippo et al. Mediators Inflamm. 2006.

Abstract

This study has evaluated whether systemic changes of plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) parallel the adhesions development and whether they could be used as predictors of adhesion risk. This has been studied in an animal model of post-surgical peritoneal adhesion by monitoring for 10 days the plasma and tissue levels of t-PA and PAI-1. The results showed that both tissular and plasmatic levels of t-PA were decreased in concomitance with the development of peritoneal adhesions. In contrast, PAI-1 was found increased into the tissue and into the plasma samples of the rats taken at 5 and 10 days time points. Inflammatory mediators such as ICAM-1, VCAM-1, and IL-6 within the peritoneal lavage fluid also correlated with the adhesion formation process. In conclusion, post-surgical peritoneal adhesions provide alterations of local inflammatory components and local and systemic fibrinolytic components, possibly with PAI-1 quenching t-PA. This may have potential for the identification of high-risk patients.

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Figures

Figure 1
Figure 1
t-PA levels in rats (n = 8) subjected to experimental peritoneal adhesion development. The time is expressed in days. Significant differences from time 0 values are expressed as *P < .01; **P < .001.
Figure 2
Figure 2
PAI-1 levels in rats (n = 8) subjected to experimental peritoneal adhesion development. The time is expressed in days. Significant differences from time 0 values are expressed as *P < .05; **P < .01.
Figure 3
Figure 3
Plasma F1 + 2 and von Willebrand levels in rats (n = 8) subjected to experimental peritoneal adhesion development. The time is expressed in days. Significant differences from time 0 values are expressed as *P < .01; **P < .001.
Figure 4
Figure 4
Levels of IL-6, VCAM-1, and ICAM-1 within the peritoneal lavage fluid of rats (n = 8) during the development of peritoneal adhesions. The time is expressed in days. Difference versus time 0 is represented as *P < .001.
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