Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Feb 1;273 ( Pt 3)(Pt 3):777-82.
doi: 10.1042/bj2730777.

The use of thioglycollate to demonstrate DNA AP (apurinic/apyrimidinic-site) lyase activities. Biological consequences of thiol addition to the 5' product of a beta-elimination reaction at an AP site in DNA

S Bricteux-Grégoire  1 , W G Verly
Affiliations

The use of thioglycollate to demonstrate DNA AP (apurinic/apyrimidinic-site) lyase activities. Biological consequences of thiol addition to the 5' product of a beta-elimination reaction at an AP site in DNA

S Bricteux-Grégoire et al. Biochem J. .

Abstract

Thioglycollate reacts with the 5' product of AP lyase activity on apurinic/apyrimidinic (AP) sites in DNA. The 3'-terminal thioglycollate-unsaturated sugar 5-phosphate adduct can be released by the use of Escherichia coli endonuclease IV or endonuclease VI, and identified by DEAE-Sephadex chromatography. In contrast, the mammalian AP endonuclease is unable to excise a 3'-terminal thiol-unsaturated sugar adduct; this lesion, which must sometimes occur in vivo, might be irreparable and have pathological consequences.

PubMed Disclaimer

References

    1. J Biol Chem. 1977 May 10;252(9):2808-14 - PubMed
    1. Eur J Biochem. 1983 Jan 1;129(3):509-17 - PubMed
    1. J Biol Chem. 1975 Oct 25;250(20):8214-9 - PubMed
    1. Nucleic Acids Res. 1989 May 11;17(9):3617-8 - PubMed
    1. Biochem J. 1989 Aug 1;261(3):707-13 - PubMed

Publication types

MeSH terms