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. 1991 Jan;82(1):12-8.
doi: 10.1111/j.1349-7006.1991.tb01738.x.

A pulmonary large cell carcinoma cell line expressing neuroendocrine cell markers and human chorionic gonadotropin alpha-subunit

K Kasai  1 , T Kameya, K Kadoya, C Wada
Affiliations

A pulmonary large cell carcinoma cell line expressing neuroendocrine cell markers and human chorionic gonadotropin alpha-subunit

K Kasai et al. Jpn J Cancer Res. 1991 Jan.

Abstract

A cell line producing the neuroendocrine cell surface antigen and human chorionic gonadotropin (hCG) alpha-subunit, designated as KTA7, was established from human large cell carcinoma using a serum-free medium, ACL-3. KTA7 continued to grow in the ACL-3 medium, showing the morphological characteristics of large cell undifferentiated carcinoma. The KTA7 cells reacted with antibodies such as 6H7 and MOC1 directed against the cell surface antigens and PGP9.5 directed against a cytoplasmic protein of neuroendocrine cells but did not possess either most epithelial markers other than low-molecular-weight keratin (Cytokeratin) or neuron-specific enolase. The KTA7 cells, by immunostaining with anti-hCG subunit antibodies, were shown to produce hCG alpha- but not beta-subunit. Northern blot analysis showed KTA7 RNA to synthesize hCG alpha-subunit mRNA but not that of the hCG beta-subunit. Thus, the hCG alpha-subunit alone was independently expressed in KTA7. Chromosome analysis showed loss of alleles of chromosome 3p and 17 in KTA7 cells but not loss of 13q. KTA7 was considered to be derived from large cell undifferentiated carcinoma with neuroendocrine differentiation (large cell neuroendocrine tumor) and thus may fine use in studies on the pathobiology of large cell-type neuroendocrine tumors since it expresses at the same time marker substances of neuroendocrine differentiation and the hCG alpha-subunit.

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References

    1. ) Kameya , T. , Shimosato , Y. , Kodama , T. , Tsumuraya , M. , Koide , T. , Yamaguchi , K. and Abe , K.Peptide hormone production by adenocarcinomas of the lung; its morphologic basis and histogenetic considerations . Virchows Arch, A , 400 , 245 – 257 ( 1983. ). - PubMed
    1. ) Shimosato , Y.Pulmonary neoplasms . In “ Diagnostic Surgical Pathology ,” ed. Sternberg S. S. , pp. 785 – 827 ( 1989. ). Raven Press; , New York .
    1. ) Kasai , K. , Kameya , T. , Okuda , T. , Terasaki , P. I. and Iwaki , Y.Immunohistochemical examination of lung cancers using monoclonal antibodies reacting with sialosylates Lewisx and sialosylated Lewisα . Virchows Arch. A , 410 , 253 – 261 ( 1986. ). - PubMed
    1. ) Abe , K. , Kameya , T. , Yamaguchi , K. , Kikuchi , K. , Adachi , I. , Tanaka , M. , Kimura , S. , Kodama , T. , Shimosato , Y. and Ishikawa , S.Hormone‐producing lung cancer – endocrinologic studies . In “ The Endocrine Lung in Health and Disease ,” ed. Becker K. I. and Gazdar A. E. , pp. 549 – 595 ( 1984. ). Saunders; , Philadelphia .
    1. ) Negatsu , T. , Ichinose , H. , Kojima , K. , Kameya , T. , Shimase , J. , Kodama , T. and Shimosato , Y.Aromatic l‐aminoaciddecarboxylaseactivitiesinhuman lung tissues: comparison between normal lung and lung carcinoma . Biochem. Med. , 34 , 52 – 59 ( 1985. ). - PubMed

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