Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Mar 1;88(5):1943-7.
doi: 10.1073/pnas.88.5.1943.

Construction of a uniform-abundance (normalized) cDNA library

S R Patanjali  1 , S Parimoo, S M Weissman
Affiliations

Construction of a uniform-abundance (normalized) cDNA library

S R Patanjali et al. Proc Natl Acad Sci U S A. .

Erratum in

  • Proc Natl Acad Sci U S A 1991 Jun 1;88(11):5066

Abstract

We have used a kinetic approach to construct cDNA libraries containing approximately equal representations of all sequences in a preparation of poly(A)+ RNA. Randomly primed cDNA fragments of a selected size range were cloned in lambda phage vector. Inserts were amplified by the polymerase chain reaction (PCR), denatured, and self-annealed under optimized conditions. After extensive but incomplete reannealing, the single-stranded fraction was relatively depleted of more abundant species of cDNA. Libraries of these fragments are suitable for cDNA subtraction, screening, or selection by hybridization and make it possible to detect and analyze cDNA corresponding to species of mRNA present at a low level in a small fraction of the cells in a complex tissue.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5367-71 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 - PubMed
    1. Arch Biochem Biophys. 1977 Mar;179(2):584-99 - PubMed
    1. Biochim Biophys Acta. 1975 Jan 6;378(1):18-21 - PubMed
    1. Nature. 1974 Jul 19;250(463):199-204 - PubMed

Publication types

LinkOut - more resources