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. 2006 Nov;24(11):1420-8.
doi: 10.1038/nbt1255. Epub 2006 Oct 22.

Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance

Qi-Wen Niu  1 , Shih-Shun Lin, Jose Luis Reyes, Kuan-Chun Chen, Hui-Wen Wu, Shyi-Dong Yeh, Nam-Hai Chua
Affiliations

Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance

Qi-Wen Niu et al. Nat Biotechnol. 2006 Nov.

Erratum in

  • Nat Biotechnol. 2007 Feb;25(2):254

Abstract

Plant microRNAs (miRNAs) regulate the abundance of target mRNAs by guiding their cleavage at the sequence complementary region. We have modified an Arabidopsis thaliana miR159 precursor to express artificial miRNAs (amiRNAs) targeting viral mRNA sequences encoding two gene silencing suppressors, P69 of turnip yellow mosaic virus (TYMV) and HC-Pro of turnip mosaic virus (TuMV). Production of these amiRNAs requires A. thaliana DICER-like protein 1. Transgenic A. thaliana plants expressing amiR-P69(159) and amiR-HC-Pro(159) are specifically resistant to TYMV and TuMV, respectively. Expression of amiR-TuCP(159) targeting TuMV coat protein sequences also confers specific TuMV resistance. However, transgenic plants that express both amiR-P69(159) and amiR-HC-Pro(159) from a dimeric pre-amiR-P69(159)/amiR-HC-Pro(159) transgene are resistant to both viruses. The virus resistance trait is displayed at the cell level and is hereditable. More important, the resistance trait is maintained at 15 degrees C, a temperature that compromises small interfering RNA-mediated gene silencing. The amiRNA-mediated approach should have broad applicability for engineering multiple virus resistance in crop plants.

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Comment in

  • A micropunch against plant viruses.
    García JA, Simón-Mateo C. García JA, et al. Nat Biotechnol. 2006 Nov;24(11):1358-9. doi: 10.1038/nbt1106-1358. Nat Biotechnol. 2006. PMID: 17093480 No abstract available.

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