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. 2005:103:209-22; discussion 222-4.

Effect of death-to-preservation time on donor corneal epithelium

Woodford S Van Meter  1 , Douglas G Katz, Harrison White, Robert Gayheart
Affiliations

Effect of death-to-preservation time on donor corneal epithelium

Woodford S Van Meter et al. Trans Am Ophthalmol Soc. 2005.

Abstract

Purpose: Surface disease is one of multiple variables affecting the quality of the postkeratoplasty donor cornea. Trauma to Bowman's layer before and during harvesting can denude the donor epithelium and result in epithelial defects in the donor following penetrating keratoplasty. Eye banks use death-to-preservation (DP) time intervals as long as 18 hours. This study evaluates the effects of higher DP time on the donor epithelium in storage medium and immediately following keratoplasty.

Methods: Eighty-one consecutive corneas were procured by the University of Kentucky Eye Bank, rated by one technician (H.W.), and used by one surgeon (W.S.V.) for elective penetrating keratoplasty. Donor records were retrospectively reviewed for age, DP time, and epithelial condition. All corneas were harvested and evaluated according to Eye Bank Association of America standards. Donor charts were reviewed for DP time and for condition of the epithelium in storage. Recipient charts were reviewed for epithelial defects following keratoplasty.

Results: Average DP time of all 81 donor corneas was 6:18 hours (ie, 6 hours, 18 minutes). Average DP time of 13 corneas with epithelial sloughing was 7:02 (range, 2:01 to 12:25) hours, and nine (69%) had DP time longer than 6 hours. Average DP time of 68 corneas with no sloughing was 6:09 (range, 1:59 to 11:03) hours (P < .32). Average DP of 28 recipients with epithelial defect on day 1 was 8:01 (range, 3:41 to 12:49), and average DP in 53 patients with an intact epithelium on day 1 was 5:23 (range, 1:59 to 9:46) (P < .001). The percentage of postoperative patients with epithelial defects in the graft on day 1 rose from 14% when DP was less than 4 hours to 100% when DP was greater than 10 hours. Average DP in 13 donors under age 30 was 8.3 hours.

Conclusion: DP time longer than 6 hours was more likely to result in sloughing of the donor epithelium. Care of donor epithelium prior to harvesting becomes increasingly important with DP times longer than 6 hours. Higher-than-average DP times occurred in donors under 30 years of age. Higher DP time results in an increasing likelihood of epithelial defects in the graft. Donor corneas with lower DP time may be important in penetrating keratoplasty ocular surface disease.

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Figures

FIGURE 1
FIGURE 1
Central epithelial defect in donor resulting from exposure prior to placement in storage medium.
FIGURE 2
FIGURE 2
Epithelial defect in graft on day 1 resulting from donor tissue with epithelial defect.
FIGURE 5A
FIGURE 5A
Anterior stromal haze in graft resulting from a persistent central epithelial defect in the graft.
FIGURE 5B
FIGURE 5B
A slit beam of light demonstrating subepithelial scarring in the graft.
FIGURE 3
FIGURE 3
The percentage of patients without sloughing of the donor epithelium decreases as donor death-to-preservation time increases.
FIGURE 4
FIGURE 4
The percentage of corneas without an epithelial defect on day 1 decreases as donor death-to-preservation time increases.
See this image and copyright information in PMC

References

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