Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Jul;72(7):2587-91.
doi: 10.1073/pnas.72.7.2587.

RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells

Y Aloni, M Shani, Y Reuveni

RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells

Y Aloni et al. Proc Natl Acad Sci U S A. 1975 Jul.

Abstract

We demonstrate here the usefulness of cytochalasin B enucleate cells for the study of the metabolism of cytoplasmic mRNA and for determining its half-life in animal cells. Simian virus 40 infected monkey cells in which the RNA had been labeled with [3H]uridine were enucleated, and the decay of the two prominent RNAs of simian virus 40, the 19S and 16S species, was measured by analysis on sucrose gradients. The results of these experiments, together with kinetic analysis of nuclear and cytoplasmic viral RNA, indicate a precursor-product relationship between the 19S and 16S cytoplasmic viral RNA species, which decay by first-order kinetics with a mean half-life of about 3 hr and 6 hr, respectively.

PubMed Disclaimer

References

    1. J Exp Med. 1954 Feb;99(2):167-82 - PubMed
    1. J Mol Biol. 1966 May;17(1):117-30 - PubMed
    1. Nat New Biol. 1973 May 2;243(122):2-6 - PubMed
    1. Nature. 1972 Nov 10;240(5376):100-2 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Mar;70(3):750-4 - PubMed

Publication types

LinkOut - more resources