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. 2006 Nov 1;128(43):13984-5.
doi: 10.1021/ja0636690.

A genetically encoded infrared probe

Kathryn C Schultz  1 , Lubica Supekova, Youngha Ryu, Jianming Xie, Roshan Perera, Peter G Schultz
Affiliations

A genetically encoded infrared probe

Kathryn C Schultz et al. J Am Chem Soc. .

Abstract

An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved that makes it possible to selectively and efficiently incorporate para-cyanophenylalanine (pCNPhe) into proteins in E. coli at sites specified by the amber nonsense codon, TAG. Substitution of pCNPhe for histidine-64 in myoglobin (Mb) affords a sensitive vibrational probe of ligand binding. This methodology provides a useful infrared reporter of protein structure, biomolecular interactions, and conformational changes.

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