Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids
- PMID: 1706708
- PMCID: PMC207792
- DOI: 10.1128/jb.173.7.2393-2397.1991
Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids
Abstract
Minireplicons from the I-complex plasmids R387 (IncK) and pIE545 (IncZ) were constructed, and the nucleotide sequences of their replication regions were compared with that of the B plasmid, pMU720. The coding sequence of the putative replication protein, RepA, of each plasmid was located. RepA of K and B plasmids were homologous, whereas RepA of Z resembled RepA1 of FII plasmid. Sequences upstream of RepA were conserved in the three I-complex plasmids. Group B and Z plasmids were incompatible.
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