Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Oct 26:6:94.
doi: 10.1186/1471-2180-6-94.

The biochemical differentiation of Enterobacter sakazakii genotypes

Carol Iversen  1 , Mike Waddington, Jim J Farmer 3rd, Stephen J Forsythe
Affiliations

The biochemical differentiation of Enterobacter sakazakii genotypes

Carol Iversen et al. BMC Microbiol. .

Abstract

Background: Enterobacter sakazakii is an emergent pathogen that has been associated with neonatal infections through contaminated powdered infant milk formula. The species was defined by Farmer et al. (1980) who described 15 biogroups according to the biochemical characterization of 57 strains. This present study compares genotypes (DNA cluster groups based on partial 16S rDNA sequence analysis) with the biochemical traits for 189 E. sakazakii strains.

Results: Analysis of partial 16S rDNA sequences gave 4 well defined phylogenetic groups. Cluster group 1 was composed of the majority of strains (170/189) and included Biogroups 1-5, 7-9, 11, 13 and 14. Cluster 3 corresponded with Biogroup 15 and cluster 4 with Biogroups 6, 10 and 12. Cluster group 2 comprised a new Biogroup 16. For the isolates in this study, the four DNA cluster groups can be distinguished using the inositol, dulcitol and indole tests.

Conclusion: This study demonstrates an agreement between genotyping (partial 16S rDNA) and biotyping and describes a new biogroup of E. sakazakii.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Maximum parsimony tree (unrooted) showing the four genomic clusters of E. sakazakii based on partial 16S rDNA sequence analysis. Gaps were not considered an extra state, and the topology was optimised using simulated annealing.
See this image and copyright information in PMC

References

    1. Iversen C, Forsythe SJ. Risk profile of Enterobacter sakazakii, an emergent pathogen associated with infant milk formula. Trends Food Sci Technol. 2003;14:443–454. doi: 10.1016/S0924-2244(03)00155-9. - DOI
    1. Muytjens HL, Zanen HC, Sonderkamp HJ, Kollee LA, Wachsmuth IK, Farmer JJ. Analysis of eight cases of neonatal meningitis and sepsis due to Enterobacter sakazakii. J Clin Microbiol. 1983;18:115–120. - PMC - PubMed
    1. Simmons BP, Gelfand MS, Haas M, Metts L, Ferguson J. Enterobacter sakazakii infections in neonates associated with intrinsic contamination of a powdered infant formula. Inf Cont Hosp Epidemiol. 1989;10:398–401. - PubMed
    1. Himelright I, Harris E, Lorch V, Anderson M. Enterobacter sakazakii infections associated with the use of powdered infant formula – Tennessee, 2001. J Amer Med Assoc. 2002;287:2204–2205. doi: 10.1001/jama.287.17.2204. - DOI - PubMed
    1. Farmer JJ, III, Asbury MA, Hickman FW, Brenner DJ, and the Enterobacteriaceae Study Group Enterobacter sakazakii: a new species of "Enterobacteriaceae" isolated from clinical specimens. Int J Syst Bacteriol. 1980;30:569–584.

Publication types

LinkOut - more resources