Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006;8(5):R62.
doi: 10.1186/bcr1614.

Predicting a local recurrence after breast-conserving therapy by gene expression profiling

Dimitry S A Nuyten  1 , Bas Kreike, Augustinus A M Hart, Jen-Tsan Ashley Chi, Julie B Sneddon, Lodewyk F A Wessels, Hans J Peterse, Harry Bartelink, Patrick O Brown, Howard Y Chang, Marc J van de Vijver
Affiliations

Predicting a local recurrence after breast-conserving therapy by gene expression profiling

Dimitry S A Nuyten et al. Breast Cancer Res. 2006.

Abstract

Introduction: To tailor local treatment in breast cancer patients there is a need for predicting ipsilateral recurrences after breast-conserving therapy. After adequate treatment (excision with free margins and radiotherapy), young age and incompletely excised extensive intraductal component are predictors for local recurrence, but many local recurrences can still not be predicted. Here we have used gene expression profiling by microarray analysis to identify gene expression profiles that can help to predict local recurrence in individual patients.

Methods: By using previously established gene expression profiles with proven value in predicting metastasis-free and overall survival (wound-response signature, 70-gene prognosis profile and hypoxia-induced profile) and training towards an optimal prediction of local recurrences in a training series, we establish a classifier for local recurrence after breast-conserving therapy.

Results: Validation of the different gene lists shows that the wound-response signature is able to separate patients with a high (29%) or low (5%) risk of a local recurrence at 10 years (sensitivity 87.5%, specificity 75%). In multivariable analysis the classifier is an independent predictor for local recurrence.

Conclusion: Our findings indicate that gene expression profiling can identify subgroups of patients at increased risk of developing a local recurrence after breast-conserving therapy.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Local recurrence Wound-signature correlation plot for the training set. Red arrow indicates optimal threshold.
Figure 2
Figure 2
Scheme for training and validation, including Kaplan–Meier curves for local-recurrence-free survival.
See this image and copyright information in PMC

References

    1. Sarrazin D, Le MG, Arriagada R, Contesso G, Fontaine F, Spielmann M, Rochard F, Le Chevalier T, Lacour J. Ten-year results of a randomized trial comparing a conservative treatment to mastectomy in early breast cancer. Radiother Oncol. 1989;14:177–184. doi: 10.1016/0167-8140(89)90165-5. - DOI - PubMed
    1. Blichert-Toft M, Rose C, Andersen JA, Overgaard M, Axelsson CK, Andersen KW, Mouridsen HT. Danish randomized trial comparing breast conservation therapy with mastectomy: six years of life-table analysis. Danish Breast Cancer Cooperative Group. J Natl Cancer Inst Monogr. 1992;11:19–25. - PubMed
    1. Veronesi U, Marubini E, Mariani L, Galimberti V, Luini A, Veronesi P, Salvadori B, Zucali R. Radiotherapy after breast-conserving surgery in small breast carcinoma: long-term results of a randomized trial. Ann Oncol. 2001;12:997–1003. doi: 10.1023/A:1011136326943. - DOI - PubMed
    1. Mariani L, Salvadori B, Marubini E, Conti AR, Rovini D, Cusumano F, Rosolin T, Andreola S, Zucali R, Rilke F, Veronesi U. Ten year results of a randomised trial comparing two conservative treatment strategies for small size breast cancer. Eur J Cancer. 1998;34:1156–1162. doi: 10.1016/S0959-8049(98)00137-3. - DOI - PubMed
    1. Veronesi U, Salvadori B, Luini A, Greco M, Saccozzi R, del Vecchio M, Mariani L, Zurrida S, Rilke F. Breast conservation is a safe method in patients with small cancer of the breast. Long-term results of three randomised trials on 1,973 patients. Eur J Cancer. 1995;31A:1574–1579. doi: 10.1016/0959-8049(95)00271-J. - DOI - PubMed

Publication types

MeSH terms