Quantitative study of the tectally projecting retinal ganglion cells in the adult frog: I. The size of the contralateral and ipsilateral projections

Abstract

The proportion of ganglion cells connected to the several central targets of the retinal projection varies in different species. In the frog, the retinotectal projection is clearly the largest branch of the optic pathway and the relative size of the tectally projecting population can be expected to be correspondingly great. However, there have been no studies aimed at quantifying the size of this population and at partitioning its contralateral and ipsilateral components. We injected the tectum with horseradish peroxidase (HRP) dried onto fine needles to count the numbers of retinal ganglion cells labeled by retrograde transport. The retinas were prepared as flat-mounts to facilitate the cell counting. The tecta were injected either unilaterally or bilaterally in mirror-symmetric loci. Specimens included completely normal frogs and frogs which had undergone unilateral optic nerve regeneration, although only normal retinas are presented in the current study. The retrograde transport interval was varied progressively (from 3 to 5 days), and single or multiple injections of HRP were placed singly or as clusters, in order to increment the cell counts toward a level of saturation. Approximately 70.9% of the neurons in the ganglion cell layer could be labeled by this method. Correcting for the presence of displaced amacrine cells, estimated to comprise approximately 16% of the neurons in the ganglion cell layer (Scalia et al., '85, Brain Res. 344:267-280), we calculate that approximately 84.4% of the retinal ganglion cells project contralaterally to the optic tectum. Flat-mounted retinas ipsilateral to unilaterally injected tecta of completely normal frogs were also examined for labeled cells. The results of injections in the rostrolateral, caudomedial, and caudolateral tectum were studied. We found that ipsilaterally labeled cells comprised no more than 2.3% of the overall population of ganglion cells in the ganglion cell layer. The ipsilaterally projecting cells were found in loci which were approximately mirror-symmetric to the regions of maximal cell labeling in the contralateral retinas from the same animals. The ipsilateral population was always displaced toward the periphery of the retina with respect to the contralateral population, regardless of whether the contralateral locus was centered in the temporal, ventronasal, or dorsonasal sector of the retina. Because the ipsilaterally projecting ganglion cells form such a minor population, and because they exist in the monocular as well as the binocular parts of the retina, it seems likely that they may not play a significant role in visual function in the frog.