Association of antitumor antibiotics, mithramycin and chromomycin, with Zn(II)

Abstract

Chromomycin A(3) (CHR) and mithramycin (MTR), members of the aureolic acid anticancer antibiotics, supposedly act by inhibiting transcription via reversible association with DNA. The complex(es) with bivalent cation such as Mg(2+) and Zn(2+) is (are) the DNA-binding ligand(s). In this paper, we report a detailed study of the association of these antibiotics with the biologically important bivalent cation, Zn(2+), because the zinc chelating ability of the antibiotics has therapeutic potential in the treatment of diseases relating to zinc dyshomeostasis. Spectroscopic methods such as absorbance, fluorescence, and circular dichroism and NMR spectroscopy have been used to characterize and understand the mechanism of complex formation. Our data show that both antibiotics form a single complex with Zn(2+) in the mole ratio of 2:1 in terms of antibiotic:Zn(2+) with an apparent binding affinity in the micro molar range. The complex has been characterized as [(D)(2)Zn(2+)] (where 'D' stands for the antibiotic). The kinetics study of the complex formation between the antibiotic(s) and Zn(2+) suggests the following mechanism: [reaction: see text] Isothermal calorimetric titration has shown that the association is entropy driven, implying the role of water molecules in complex formation. (1)H NMR spectroscopic data of the complex favor a tetrahedral arrangement around the Zn(2+) ion with the antibiotic acting as a bidentate ligand.