Direct cleavage of a DNA fragment by a bleomycin-oligonucleotide derivative

Abstract

Bleomycin A5 oligonucleotide derivative was used for direct cleavage of a DNA target. In the presence of Fe2+ ions and 2-mercaptoethanol, Blm-R-pd(CCAAACA) (I) damaged the target. pd(TGTTTGGCGAAGGA), with the yield of 80% , without affecting its own oligonucleotide tail. The sites of cleavage were T3-T5 and G6-G7. Unbound bleomycin A5 damaged the G6-G7-C8 site. Reagent I formed more stable complementary complexes with the target than parent oligonucleotide (ATm = 11 C).