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. 1991 Mar 25;280(2):271-3.
doi: 10.1016/0014-5793(91)80309-q.

Direct cleavage of a DNA fragment by a bleomycin-oligonucleotide derivative

D S Sergeyev  1 , T S Godovikova, V F Zarytova
Affiliations
Free article

Direct cleavage of a DNA fragment by a bleomycin-oligonucleotide derivative

D S Sergeyev et al. FEBS Lett. .
Free article

Abstract

Bleomycin A5 oligonucleotide derivative was used for direct cleavage of a DNA target. In the presence of Fe2+ ions and 2-mercaptoethanol, Blm-R-pd(CCAAACA) (I) damaged the target. pd(TGTTTGGCGAAGGA), with the yield of 80% , without affecting its own oligonucleotide tail. The sites of cleavage were T3-T5 and G6-G7. Unbound bleomycin A5 damaged the G6-G7-C8 site. Reagent I formed more stable complementary complexes with the target than parent oligonucleotide (ATm = 11 C).

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