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. 2006 Nov 1;62(Pt 11):1100-3.
doi: 10.1107/S1744309106038887. Epub 2006 Oct 20.

New crystal forms of Diocleinae lectins in the presence of different dimannosides

Frederico Bruno Mendes Batista Moreno  1 , Gustavo Arruda Bezerra, Taianá Maia de Oliveira, Emmanuel Prata de Souza, Bruno Anderson Matias da Rocha, Raquel Guimarães Benevides, Plínio Delatorre, Benildo Sousa Cavada, Walter Filgueira de Azevedo Jr
Affiliations

New crystal forms of Diocleinae lectins in the presence of different dimannosides

Frederico Bruno Mendes Batista Moreno et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Studying the interactions between lectins and sugars is important in order to explain the differences observed in the biological activities presented by the highly similar proteins of the Diocleinae subtribe. Here, the crystallization and preliminary X-ray data of Canavalia gladiata lectin (CGL) and C. maritima lectin (CML) complexed with Man(alpha1-2)Man(alpha1)OMe, Man(alpha1-3)Man(alpha1)OMe and Man(alpha1-4)Man(alpha1)OMe in two crystal forms [the complexes with Man(alpha1-3)Man(alpha1)OMe and Man(alpha1-4)Man(alpha1)OMe crystallized in space group P3(2) and those with Man(alpha1-2)Man(alpha1)OMe crystallized in space group I222], which differed from those of the native proteins (P2(1)2(1)2 for CML and C222 for CGL), are reported. The crystal complexes of ConA-like lectins with Man(alpha1-4)Man(alpha1)OMe are reported here for the first time.

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Figures

Figure 1
Figure 1
Crystals of (a) CGL–Man(α1-2)Man(α1)OMe, (b) CML–Man(α1-2)Man(α1)OMe, (c) CGL–Man(α1-3)Man(α1)OMe, (d) CML–Man(α1-3)Man(α1)OMe, (e) CGL–Man(α1-4)Man(α1)OMe and (f) CML–Man(α1-4)Man(α1)OMe.
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