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. 1990 Dec;10(4):553-8.
doi: 10.1007/BF00712848.

Picomolar-affinity binding and inhibition of adenylate cyclase activity by melatonin in Syrian hamster hypothalamus

L P Niles  1 , F Hashemi
Affiliations

Picomolar-affinity binding and inhibition of adenylate cyclase activity by melatonin in Syrian hamster hypothalamus

L P Niles et al. Cell Mol Neurobiol. 1990 Dec.

Abstract

1. The effect of melatonin on forskolin-stimulated adenylate cyclase activity was measured in homogenates of Syrian hamster hypothalamus. In addition, the saturation binding characteristics of the melatonin receptor ligand, [125I]iodomelatonin, was examined using an incubation temperature (30 degrees C) similar to that used in enzyme assays. 2. At concentrations ranging from 10 pM to 1 nM, melatonin caused a significant decrease in stimulated adenylate cyclase activity with a maximum inhibition of approximately 22%. 3. Binding experiments utilizing [125I]iodomelatonin in a range of approximately 5-80 pM indicated a single class of high-affinity sites: Kd = 55 +/- 9 pM, Bmax = 1.1 +/- 0.3 fmol/mg protein. 4. The ability of picomolar concentrations of melatonin to inhibit forskolin-stimulated adenylate cyclase activity suggests that this affect is mediated by picomolar-affinity receptor binding sites for this hormone in the hypothalamus.

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References

    1. Brown, G. M., and Niles, L. P. (1982). Studies on melatonin and other pineal factors. InClinical Neuroendocrinology, Vol. II (G. M. Besser and L. Martini, Eds.), Academic Press, New York, pp. 205–264.
    1. Carlson, L. L., Weaver, D. R., and Reppert, S. M. (1989). Melatonin signal transduction in hamster brain: Inhibition of adenylyl cyclase by a pertussis toxin-sensitive G protein.Endocrinology1252670–2676. - PubMed
    1. Duncan, M. J., Takahashi, J. S., and Dubocovich, M. L. (1988). 2-[125I]Iodomelatonin binding sites in hamster brain membranes: Pharmacological characteristics and regional distribution.Endocrinology1221825–1833. - PubMed
    1. Duncan, M. J., Takahashi, J. S., and Dubocovich, M. L. (1989). Characteristics and autoradiographic localization of 2-[125I]iodomelatonin binding sites in Djungarian hamster brain.Endocrinology1251011–1018. - PubMed
    1. Katada T., Gilman, A. G., Watanabe, Y., Bower, S., and Jakobs, K. H. (1985). Protein kinase C phosphorylates the inhibitory guanine nucleotide binding regulatory component and apparently suppresses its function in hormonal inhibition of adenylate cylase.Eur. J. Biochem.151431–437. - PubMed

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