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. 2006 Nov;13(11):1291-3.
doi: 10.1128/CVI.00158-06.

Mycobacterium tuberculosis malate synthase- and MPT51-based serodiagnostic assay as an adjunct to rapid identification of pulmonary tuberculosis

Jacqueline M Achkar  1 , Yuxin Dong, Robert S Holzman, John Belisle, Irene S Kourbeti, Tsering Sherpa, Rany Condos, William N Rom, Suman Laal
Affiliations

Mycobacterium tuberculosis malate synthase- and MPT51-based serodiagnostic assay as an adjunct to rapid identification of pulmonary tuberculosis

Jacqueline M Achkar et al. Clin Vaccine Immunol. 2006 Nov.

Abstract

The 81-kDa malate synthase (MS; Rv 1837c) and the 27-kDa MPT51 (Rv 3803c) of Mycobacterium tuberculosis are immunodominant antigens recognized by serum antibodies from approximately 80% of human immunodeficiency virus-negative smear-positive tuberculosis patients from India. We now provide evidence that the use of the MS/MPT51-based serodiagnostic assay can serve as an adjunct to sputum microscopy in the rapid diagnosis of pulmonary tuberculosis.

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Figures

FIG. 1.
FIG. 1.
Detection rate of active TB in U.S. subjects by either sputum smears for AFB (smear; with one or more of the initial three samples positive), serum Ab reactivity to MS and/or MPT51 (MS/MPT51), or both methods combined (smear+MS/MPT51). Control groups are healthy, HIV-negative volunteers (HIV−/TB−; 36 TST positive and 19 TST negative) and asymptomatic HIV-infected patients (HIV+/TB−). Patient groups with culture-confirmed PTB are either HIV negative (HIV−/TB+) or HIV infected (HIV+/TB+). *, P = 0.023 for comparison of sensitivity of smear+MS/MPT51 to that of smear alone.
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