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. 1975 Jun;147(3):609-12.
doi: 10.1042/bj1470609.

A kinetic method to establish the specificity of spin labelling of macromolecules

J R Griffiths, N C Price, G K Radda

A kinetic method to establish the specificity of spin labelling of macromolecules

J R Griffiths et al. Biochem J. 1975 Jun.

Abstract

A method has been devised for establishing the specificity of spin labelling of a macromolecule. Analysis of the rate of incorporation of spin label under different conditions permits the assignment of reacting groups to classes of differing reactivity. The method is illustrated for the enzyme glycogen phosphorylase b.

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