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. 2007 Jan;45(1):252-4.
doi: 10.1128/JCM.01489-06. Epub 2006 Nov 8.

Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Leen Rigouts  1 , Oscar Nolasco, Pim de Rijk, Elie Nduwamahoro, Armand Van Deun, Andy Ramsay, Jorge Arevalo, Françoise Portaels
Affiliations

Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Leen Rigouts et al. J Clin Microbiol. 2007 Jan.

Abstract

New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.

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Figures

FIG. 1.
FIG. 1.
Location of the newly developed primers on the M. tuberculosis rpoB gene relevant to previously used primers for nested PCR (15) and relevant to the currently known mutations conferring rifampin resistance. Codon designation is in accordance with the M. tuberculosis nomenclature (7). Cl., cluster.
See this image and copyright information in PMC

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