Locus coeruleus activation by foot shock or electrical stimulation inhibits amygdala neurons

Abstract

The noradrenergic nucleus locus coeruleus (LC) has a direct projection to the basal lateral amygdala (BLA). Behavioral, lesion and pharmacological studies suggest that this pathway has an important role in mediating responses to emotional stimuli and in the formation of long term memory. The effect of LC activation on the activity of BLA neurons in vivo is not known. Therefore, in the present experiments, simultaneous extracellular unit recordings were made in the two regions while the anesthetized rat received electrical stimulation of the paw to simulate a real-life acute stressor, commonly used as an aversive reinforcer in conditioning experiments. All LC neurons exhibited a multiphasic excitatory response followed by prolonged inhibition. Responses of BLA cells were more heterogeneous, but predominantly inhibitory, with a release from inhibition during the refractory phase of LC. Direct electrical stimulation of the LC with a single pulse also elicited an inhibitory response in BLA. BLA response to both footshock and LC stimulation was partially blocked by the beta adrenergic receptor antagonist, timolol, infused into the BLA. These experiments are the first to report in vivo effects of activation of the noradrenergic system on neuronal activity in the BLA.