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Comparative Study
. 1991 May 15;88(10):4080-3.
doi: 10.1073/pnas.88.10.4080.

Effects of metal ions, including Mg2+ and lanthanides, on the cleavage of ribonucleotides and RNA model compounds

R Breslow  1 , D L Huang
Affiliations
Comparative Study

Effects of metal ions, including Mg2+ and lanthanides, on the cleavage of ribonucleotides and RNA model compounds

R Breslow et al. Proc Natl Acad Sci U S A. .

Abstract

The cyclization/cleavage of 3',5'-uridyluridine to form 2',3'-cyclic uridylic acid is very effectively catalyzed by Eu3+, and the cyclization/cleavage of the 1-p-nitrophenyl phosphate ester of propane-1,2-diol also shows strong metal ion catalysis by Eu3+, Tb3+, and Yb3+. It also shows moderate catalysis by Mg2+, but not by Ca2+; Zn2+ and Pb2+ are also good catalysts. Various ligands activate these reactions further, and imidazole apparently acts as an additional base catalyst. Some cyclodextrin derivatives act to bind both the substrate and the metal ion but, contrary to what is reported elsewhere, there is no strong selectivity among nucleotides that can be ascribed to cyclodextrin binding.

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References

    1. Biochem Z. 1954;326(2):89-96 - PubMed
    1. Science. 1991 Jan 25;251(4992):401-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Mar;86(6):1746-50 - PubMed
    1. Adv Enzymol Relat Areas Mol Biol. 1989;62:1-36 - PubMed
    1. Biopolymers. 1965;3(1):79-94 - PubMed

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