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. 2007 May 15;22(11):2570-6.
doi: 10.1016/j.bios.2006.10.009. Epub 2006 Nov 13.

Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

Bella Shlyahovsky  1 , Di Li, Eugenii Katz, Itamar Willner
Affiliations

Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

Bella Shlyahovsky et al. Biosens Bioelectron. .

Abstract

Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H(2)O(2), and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H(2)O(2).

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