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. 1977 Mar;4(3):685-95.
doi: 10.1093/nar/4.3.685.

Polyamine induced aggregation of DNA

A Osland, K Kleppe
Free PMC article

Polyamine induced aggregation of DNA

A Osland et al. Nucleic Acids Res. 1977 Mar.
Free PMC article

Abstract

Polyamine induced aggregation of various DNAs has been studied under conditions usually employed in many enzymatic assays where DNA is one of the substrates. Spermine was by far the most efficient polyamine in causing aggregation followed by spermidine and cadaverine. All double-stranded and naturally occurring single-stranded DNAs were found to aggregate. No aggregation of single-stranded homodeoxypolymers could be detected under the same conditions. The concentration of polyamine at which the aggregation commenced was found to be a linear function of the DNA concentration. The slope of the curves depended on the nature of the polyamine, DNA the concentration of Mg++ and the ionic strength.

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