Enzymatic and non-enzymatic assay of superoxide dismutase
- PMID: 171001
- DOI: 10.1016/s0300-9084(75)80147-7
Enzymatic and non-enzymatic assay of superoxide dismutase
Abstract
Superoxide dismutase from breef brain and rat liver was assayed in an enzymatic system, using xanthine oxidase, and a non-enzymatic system, based on aerobic reduction of nitro-blue tetrazolium in presence of phenazine methosulphate. The non-enzymatic assay is rapid and simple and permits simulatneous analysis of many samples. Similar results are found by the two methods of assay of superoxide dismutase.
Similar articles
-
On the mechanism of production of superoxide radical by reaction mixtures containing NADH, phenazine methosulfate, and nitroblue tetrazolium.Arch Biochem Biophys. 1984 Jan;228(1):155-8. doi: 10.1016/0003-9861(84)90056-0. Arch Biochem Biophys. 1984. PMID: 6320732
-
Studies on the reduction of nitroblue tetrazolium chloride mediated through the action of NADH and phenazine methosulphate.Chem Biol Interact. 1978 Dec;23(3):281-91. doi: 10.1016/0009-2797(78)90090-x. Chem Biol Interact. 1978. PMID: 214250
-
Microplate superoxide dismutase assay employing a nonenzymatic superoxide generator.Anal Biochem. 1995 Dec 10;232(2):243-8. doi: 10.1006/abio.1995.0014. Anal Biochem. 1995. PMID: 8747482
-
The involvement of superoxide anions in the nitro blue tetrazolium chloride reduction mediated by NADH and phenazine methosulfate.Anal Biochem. 1989 Jan;176(1):170-4. doi: 10.1016/0003-2697(89)90288-1. Anal Biochem. 1989. PMID: 2540673
-
Reduction of mercuric ion in vitro by superoxide anion.Physiol Chem Phys Med NMR. 2002;34(2):185-9. Physiol Chem Phys Med NMR. 2002. PMID: 12841335
Cited by
-
Anticancer activity of Ilex khasiana, a rare and endemic species of holly in Northeast India, against murine lymphoma.Heliyon. 2025 Jan 10;11(2):e41839. doi: 10.1016/j.heliyon.2025.e41839. eCollection 2025 Jan 30. Heliyon. 2025. PMID: 39885875 Free PMC article.
-
Biochemical and Antioxidant Characteristics of Chlorococcum oleofaciens (Chlorophyceae, Chlorophyta) under Various Cultivation Conditions.Plants (Basel). 2024 Aug 29;13(17):2413. doi: 10.3390/plants13172413. Plants (Basel). 2024. PMID: 39273897 Free PMC article.
-
Ginkgo biloba extract inhibits oxygen species production generated by phorbol myristate acetate stimulated human leukocytes.Experientia. 1987 Feb 15;43(2):181-4. doi: 10.1007/BF01942843. Experientia. 1987. PMID: 3028858
-
Antioxidant Status and Biotechnological Potential of New Vischeria vischeri (Eustigmatophyceae) Soil Strains in Enrichment Cultures.Antioxidants (Basel). 2023 Mar 6;12(3):654. doi: 10.3390/antiox12030654. Antioxidants (Basel). 2023. PMID: 36978902 Free PMC article.
-
Deletion of chromosome 21 and normal intelligence: molecular definition of the lesion.Hum Genet. 1991 Jun;87(2):112-8. doi: 10.1007/BF00204163. Hum Genet. 1991. PMID: 2066097
MeSH terms
Substances
LinkOut - more resources
Full Text Sources