Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 May 25;19(10):2629-35.
doi: 10.1093/nar/19.10.2629.

2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event

M Cotten  1 , B Oberhauser, H Brunar, A Holzner, G Issakides, C R Noe, G Schaffner, E Wagner, M L Birnstiel
Affiliations
Free PMC article

2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event

M Cotten et al. Nucleic Acids Res. .
Free PMC article

Abstract

We describe the synthesis of 2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides and demonstrate their utility as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event. These 2'-O-modified compounds were designed to possess the binding affinity of an RNA molecule towards a complementary RNA target with an enhanced stability against nucleases. The 2'-O-methyl and 2'-O-ethyl antisense compounds function as potent inhibitors of the reaction at 1-10 nM, approximately 5-fold more effective than a natural antisense RNA molecule and requiring an approximate 5-fold excess over the target RNA for 80% inhibition of the processing reaction.

PubMed Disclaimer

References

    1. Nature. 1986 Oct 30-Nov 5;323(6091):777-81 - PubMed
    1. EMBO J. 1986 Jun;5(6):1319-26 - PubMed
    1. Pharm Res. 1988 Sep;5(9):539-49 - PubMed
    1. Cell. 1989 Nov 3;59(3):531-9 - PubMed
    1. Mol Cell Biol. 1989 Oct;9(10):4479-87 - PubMed

MeSH terms