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. 2006 Nov 22;128(46):14768-9.
doi: 10.1021/ja065835+.

A cellular FRET-based sensor for beta-O-GlcNAc, a dynamic carbohydrate modification involved in signaling

Luz D Carrillo  1 , Lakshmipriya Krishnamoorthy, Lara K Mahal
Affiliations

A cellular FRET-based sensor for beta-O-GlcNAc, a dynamic carbohydrate modification involved in signaling

Luz D Carrillo et al. J Am Chem Soc. .

Abstract

beta-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.

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