[A micromodification of the method of determining the activity of processes of free radical oxidation]

Abstract

A micromodification of the method for registration of bivalent iron ion-initiated chemiluminescence of the apo-beta-lipoprotein total fraction isolated from the blood serum is described. A significant elevation of lipid peroxide levels and of lipid ability to oxidation, paralleled by reduced levels of endogenous antioxidants as against the norm were revealed in the patients with acute disorders of brain circulation with the use of this method. Chemiluminescent studies of lipid peroxidation processes permit monitoring lipid peroxidation reactions and thus obtain a complex of parameters that characterize the status of these processes in the studied system. Due to its high sensitivity and specificity, chemiluminescent analysis may become one of the principal methods in studies of lipid peroxidation processes.