Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Jan;45(1):257-8.
doi: 10.1128/JCM.01552-06. Epub 2006 Nov 15.

Use of partial 16S rRNA gene sequencing for identification of Legionella pneumophila and non-pneumophila Legionella spp

D A Wilson  1 , U Reischl, G S Hall, G W Procop
Affiliations

Use of partial 16S rRNA gene sequencing for identification of Legionella pneumophila and non-pneumophila Legionella spp

D A Wilson et al. J Clin Microbiol. 2007 Jan.

Abstract

We examined 49 Legionella species, 26 L. pneumophila and 23 non-pneumophila Legionella spp., using partial 16S rRNA gene sequencing. This approach accurately identified all the L. pneumophila isolates, characterized all non-pneumophila Legionella isolates as such within this genus, and classified most (20/23; 87%) of the non-pneumophila Legionella isolates to the species level.

PubMed Disclaimer

References

    1. Pedro-Botet, M. L., and M. Sabria. 2005. Legionellosis. Semin. Respir. Crit. Care Med. 26:625-634. - PubMed
    1. Taylor, T. H., and M. A. Albrecht. 1995. Legionella bozemanii cavitary pneumonia poorly responsive to erythromycin: case report and review. Clin. Infect. Dis. 20:329-334. - PubMed
    1. Reischl, U., H.-J. Linde, N. Lehn, O. Landt, K. Barratt, and N. Wellinghausen. 2002. Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis. J. Clin. Microbiol. 40:3814-3817. - PMC - PubMed
    1. Hayden, R. T., J. R. Uhl, X. Qian, M. K. Hopkins, M. C. Aubry, A. H. Limper. R. V. Lloyd, and F. R. Cockerill. 2001. Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture. J. Clin. Microbiol. 39:2618-2626. - PMC - PubMed

Publication types

LinkOut - more resources