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. 2007 Jan;4(1):35-7.
doi: 10.1038/nmeth977. Epub 2006 Nov 19.

Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Arne Hansen  1 , Yidong Chen, Jason M Inman, Quang N Phan, Zhi-Qing Qi, Charlie C Xiang, Miklos Palkovits, Natasha Cherman, Sergei A Kuznetsov, Pamela G Robey, Eva Mezey, Michael J Brownstein
Affiliations

Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Arne Hansen et al. Nat Methods. 2007 Jan.

Abstract

G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

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