doi: 10.1263/jbb.102.362.
Analysis of essential amino acid residues for catalytic activity of glutaminase from Micrococcus luteus K-3
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- PMID: 17116585
- DOI: 10.1263/jbb.102.362
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Analysis of essential amino acid residues for catalytic activity of glutaminase from Micrococcus luteus K-3
J Biosci Bioeng.
2006 Oct.
Abstract
Structural-based mutational analysis of salt-tolerant glutaminase from Micrococcus luteus K-3 (Micrococcus glutaminase) revealed that three amino acid residues, S64, K67, and E160, were essential to a catalytic reaction. The result suggested that Micrococcus glutaminase had a possible catalytic mechanism similar to class A beta-lactamase rather than glutaminase-asparaginase from Pseudomonas 7A.
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