Endothelial cells are required for inhibition of contractile responses induced by reduction in extracellular magnesium and sodium ions in rat aortic smooth muscle [corrected]

Abstract

The possible importance of facilitation of sodium-calcium (Na(+)-Ca2+) exchange by removal of extracellular magnesium ions ([Mg2+]o) in expression of endothelium-dependent relaxation was investigated in aortic rings isolated from female rats. Simultaneous [Mg2+]o withdrawal (0 mM Mg2+) and reduction in extracellular Na+ (Total [Na+]o = 84 mM), by replacement of NaCl with isosmolar amounts of sucrose in normal Krebs-Ringer bicarbonate (NKRB), induced significant increases of basal tone of denuded rat aortic rings, but not in tissues with intact endothelium. These vascular effects were not affected by indomethacin, phentolamine or atropine in any of the tissues tested. Reintroduction of 1.2 mM Mg2+ or removal of extracellular Ca2+ ([Ca2+]o) from the Mg2+ and Na(+)-deficient incubation media induced complete relaxation of the denuded tissues. Methylene blue (10(-5) M), an inhibitor of endothelium-derived relaxant factor (EDRF), potentiated tension development in intact tissues. These results suggest that: (1) as in vascular muscle, Mg2+ plays an important role in Ca2+ homeostasis in endothelial cells (EC), probably via Na(+)-Ca2+ exchange; and (2) such Mg(+)-regulated internal Na(+)-dependent Ca2+ entry participates in the expression of endothelium-dependent relaxation.