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. 2007 Jan;4(1):39-42.
doi: 10.1038/nmeth975. Epub 2006 Nov 26.

Fast manipulation of cellular cAMP level by light in vivo

Saskia Schröder-Lang  1 , Martin Schwärzel, Reinhard Seifert, Timo Strünker, Suneel Kateriya, Jens Looser, Masakatsu Watanabe, U Benjamin Kaupp, Peter Hegemann, Georg Nagel
Affiliations

Fast manipulation of cellular cAMP level by light in vivo

Saskia Schröder-Lang et al. Nat Methods. 2007 Jan.

Abstract

The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACalpha and PACbeta. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.

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