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. 1991 Mar;343(3):334-6.
doi: 10.1007/BF00251136.

Expression of the neuronal noradrenaline transporter in Xenopus laevis oocytes

D Coppeneur  1 , B Lingen, G Sanders, M C Dabauvalle, H Bönisch
Affiliations

Expression of the neuronal noradrenaline transporter in Xenopus laevis oocytes

D Coppeneur et al. Naunyn Schmiedebergs Arch Pharmacol. 1991 Mar.

Abstract

RNA isolated from both rat phaeochromocytoma (PC12) cells and bovine adrenal medulla was size-selected and injected into oocytes of Xenopus laevis. Expression of the neuronal noradrenaline transporter was assayed 3 days after injection by measuring the nisoxetine-sensitive noradrenaline uptake. A 2 kilobase fraction of either total RNA derived from both tissues or of polyadenylated mRNA obtained from bovine adrenal medulla caused Na(+)-dependent transport of 3H-noradrenaline in the injected oocytes.

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