doi: 10.1093/nass/48.1.209.
A ligase ribozyme obtained from a structured pool
Affiliations
- PMID: 17150552
- DOI: 10.1093/nass/48.1.209
Item in Clipboard
A ligase ribozyme obtained from a structured pool
Nucleic Acids Symp Ser (Oxf).
2004.
Abstract
Here we report the in vitro selection experiment employed to construct a catalytic site on a self-folding RNA. To practice the selection, the sequence of 30 nucleotides was located in close proximity of the putative reaction site in a derivative of a naturally occurring RNA with a well known 3D structure. The acquired catalytic RNA retained the starting 3D structure and was successfully reconstructed according to its modular structure.
Similar articles
-
Generation of a catalytic module on a self-folding RNA.RNA. 2004 Dec;10(12):1900-6. doi: 10.1261/rna.7170304. Epub 2004 Nov 3. RNA. 2004. PMID: 15525711 Free PMC article.
-
Evolution in vitro of an RNA enzyme with altered metal dependence.Nature. 1993 Jan 14;361(6408):182-5. doi: 10.1038/361182a0. Nature. 1993. PMID: 8421526
-
Probing the folding landscape of the Tetrahymena ribozyme: commitment to form the native conformation is late in the folding pathway.J Mol Biol. 2001 May 18;308(5):839-51. doi: 10.1006/jmbi.2001.4751. J Mol Biol. 2001. PMID: 11352576
-
Catalytic strategies of self-cleaving ribozymes.Acc Chem Res. 2008 Aug;41(8):1027-35. doi: 10.1021/ar800050c. Epub 2008 Jul 25. Acc Chem Res. 2008. PMID: 18652494 Review.
-
When to believe what you see.Mol Cell. 2006 Aug;23(4):447-50. doi: 10.1016/j.molcel.2006.08.001. Mol Cell. 2006. PMID: 16916633 Review.
Cited by
-
Closing the circle: replicating RNA with RNA.Cold Spring Harb Perspect Biol. 2010 Oct;2(10):a002204. doi: 10.1101/cshperspect.a002204. Epub 2010 Jun 16. Cold Spring Harb Perspect Biol. 2010. PMID: 20554706 Free PMC article. Review.