doi: 10.1093/nass/49.1.51.
NMR spectroscopic analyses of functional nucleic acids-metal interaction and their solution structure analyses
Affiliations
- PMID: 17150628
- DOI: 10.1093/nass/49.1.51
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NMR spectroscopic analyses of functional nucleic acids-metal interaction and their solution structure analyses
Nucleic Acids Symp Ser (Oxf).
2005.
Abstract
We have studied complexations of functional nucleic acids and metal ions, by means of NMR spectroscopy. In the case of hammerhead ribozymes, they have a metal ion-binding motif in their core sequences. Upon the metallation of N7 of a guanosine in an RNA duplex modelled after hammerhead ribozymes, 20 ppm higher field shift of N7 was observed in 1-dimensional (1D) 15N NMR spectra. It was found that metal ion binding to nucleobases were detectable with 15N NMR spectroscopy.
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