doi: 10.1093/nass/49.1.77.
RNA interference induced by siRNAs modified with 4'-thioribonucleosides
Affiliations
- PMID: 17150641
- DOI: 10.1093/nass/49.1.77
Item in Clipboard
RNA interference induced by siRNAs modified with 4'-thioribonucleosides
Nucleic Acids Symp Ser (Oxf).
2005.
Abstract
Short interfering RNAs (siRNAs) variously modified with 4'-thioribonucleosides against the photinus luciferase gene and the renilla luciferase gene were tested for their induction of the RNA interference (RNAi) activity in cultured NIH/3T3 cells. Results indicated that modifications with 4'-thioribonucleosides on siRNA against the photinus luciferase were as potent as natural siRNA. On the other hand, modifications with 4'-thioribonucleosides on natural siRNA against the renilla luciferase improved their RNAi activity. These results suggest that 4'-thioribonucleosides might be potentially useful in the development of novel and effective chemically modified siRNAs.
Similar articles
-
RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells.FEBS Lett. 2005 Jun 6;579(14):3115-8. doi: 10.1016/j.febslet.2005.04.073. FEBS Lett. 2005. PMID: 15919084
-
Study of modification pattern-RNAi activity relationships by using siRNAs modified with 4'-thioribonucleosides.Chembiochem. 2007 Nov 23;8(17):2133-8. doi: 10.1002/cbic.200700342. Chembiochem. 2007. PMID: 17924376
-
Improving RNA interference in mammalian cells by 4'-thio-modified small interfering RNA (siRNA): effect on siRNA activity and nuclease stability when used in combination with 2'-O-alkyl modifications.J Med Chem. 2006 Mar 9;49(5):1624-34. doi: 10.1021/jm050822c. J Med Chem. 2006. PMID: 16509579
-
On the art of identifying effective and specific siRNAs.Nat Methods. 2006 Sep;3(9):670-6. doi: 10.1038/nmeth911. Nat Methods. 2006. PMID: 16929310 Review.
-
RNA interference and chemically modified small interfering RNAs.Curr Opin Chem Biol. 2004 Dec;8(6):570-9. doi: 10.1016/j.cbpa.2004.10.007. Curr Opin Chem Biol. 2004. PMID: 15556399 Review.