doi: 10.1016/0034-5288(91)90138-e.
Primary culture of chicken bursal plical epithelium
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- PMID: 1715596
- DOI: 10.1016/0034-5288(91)90138-e
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Primary culture of chicken bursal plical epithelium
Res Vet Sci.
1991 May.
Abstract
Plical epithelial cells were obtained by trypsin-EDTA treatment of chicken bursa of Fabricius and cultured in the presence of type IV collagen. The culture became confluent six to seven days after seeding. The grown cells showed a positive reaction for cytokeratin by immunostaining and had ultrastructural characteristics of the epithelial cells in vivo. The cell culture will be useful for parasitological and virological studies.
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