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. 1991 May;50(3):352-4.
doi: 10.1016/0034-5288(91)90138-e.

Primary culture of chicken bursal plical epithelium

T Nunoya  1 , M Tajima, C Itakura
Affiliations

Primary culture of chicken bursal plical epithelium

T Nunoya et al. Res Vet Sci. 1991 May.

Abstract

Plical epithelial cells were obtained by trypsin-EDTA treatment of chicken bursa of Fabricius and cultured in the presence of type IV collagen. The culture became confluent six to seven days after seeding. The grown cells showed a positive reaction for cytokeratin by immunostaining and had ultrastructural characteristics of the epithelial cells in vivo. The cell culture will be useful for parasitological and virological studies.

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