[Synthesis, cloning, and expression of artificial genes, coding antigenic determinants of the foot-and-mouth virus substrain A22]
- PMID: 1716100
[Synthesis, cloning, and expression of artificial genes, coding antigenic determinants of the foot-and-mouth virus substrain A22]
Abstract
Chemical-enzymatic synthesis and cloning in Escherichia coli of double-stranded DNAs, coding for simple and complex antigenic determinants of foot-and-mouth disease virus (FMDV) strain A22, have been carried out. The simple antigenic determinants are a part of the viral coat protein VP1 (amino acid sequence 131-152 or 131-160) whereas the complex antigenic determinants comprise additionally the amino acid sequence 200-213 of VP1 linked to N-terminus of simple antigenic determinants through a tetrapeptide spacer Pro-Pro-Ser-Pro. Recombinant DNAs containing genes for antigenic determinants of FMDV fused with C-terminus of gene for human tumor necrosis factor (hrTNF) have been constructed. Expression of the hybrid genes and properties of the proteins coded were studied. All recombinant proteins were shown to interact specifically with polyclonal antibodies both against hrTNF and FMDV strain A22. The recombinant proteins produced by bacteria are perspective for study as a vaccine against FMDV.
Similar articles
-
[Recombinant plasmids containing hybrid protein genes with antigenic determinants of the foot and mouth virus].Bioorg Khim. 1989 Apr;15(4):508-13. Bioorg Khim. 1989. PMID: 2473757 Russian.
-
[Primary structure of the DNA copy of the protein VP1 gene of the foot-and-mouth disease virus A22].Bioorg Khim. 1986 Mar;12(3):416-9. Bioorg Khim. 1986. PMID: 2421736 Russian.
-
[A new approach to DNA synthesis from synthetic oligonucleotides. Synthesis of DNA coding for the repeated antigenic determinant of foot and mouth disease virus].Bioorg Khim. 1987 Jan;13(1):69-81. Bioorg Khim. 1987. PMID: 2436628 Russian.
-
Modified-live infectious bovine rhinotracheitis virus (IBRV) vaccine expressing foot-and-mouth disease virus (FMDV) capsid protein epitopes on surface of hybrid virus particles.Adv Exp Med Biol. 1991;303:211-20. doi: 10.1007/978-1-4684-6000-1_23. Adv Exp Med Biol. 1991. PMID: 1725233 Review. No abstract available.
-
Biotechnological approach to a new foot-and-mouth disease virus vaccine.Biotechnol Genet Eng Rev. 1984;1:223-59. doi: 10.1080/02648725.1984.10647787. Biotechnol Genet Eng Rev. 1984. PMID: 6085855 Review.