Mapping the transcription site of the SV40-specific late 16 S mRNA
- PMID: 171628
- PMCID: PMC343563
- DOI: 10.1093/nar/2.10.1995
Mapping the transcription site of the SV40-specific late 16 S mRNA
Abstract
This paper describes the purification of polysomal RNA from SV40-lytically infected CV1 (monkey) cells and separation of the two distinct classes of SV40-specific mRNA sedimenting at 16 S and 19 S. These classes have been hybridized with the whole SV40 DNA genome as well as with the SV40 Hind fragments. The results have permitted the mapping of SV40-specific late 16 S mRNA from approximately 0.945 to 0.175 map units.
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