doi: 10.1073/pnas.72.9.3387.
Reversible effects of chaotropic agents on the proton permeability of Escherichia coli membrane vesicles
- PMID: 171661
- PMCID: PMC432998
- DOI: 10.1073/pnas.72.9.3387
Item in Clipboard
Reversible effects of chaotropic agents on the proton permeability of Escherichia coli membrane vesicles
Proc Natl Acad Sci U S A.
1975 Sep.
Abstract
Extraction of E. coli ML 308-225 membrane vesicles with chaotropic agents causes the vesicles to become specifically permeable to protons. As a result, the vesicles no longer generate a membrane potential, interior negative, and they do not catalyze respiration-dependent lactose or proline transport. Treatment of the extracted vesicles with various carbodiimides decreases the permeability of the vesicle membrane to protons, causing them to regain their ability to generate a membrane potential. By this means, active transport is completely reactivated. Exposure of the vesicles to carbodiimides prior to extraction with chaotropic agents makes transport activity impervious to the effects of the chaotropes.
Similar articles
-
The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles.Proc Natl Acad Sci U S A. 1976 Jun;73(6):1892-6. doi: 10.1073/pnas.73.6.1892. Proc Natl Acad Sci U S A. 1976. PMID: 6961 Free PMC article.
-
Energy transduction in Escherichia coli. The effect of chaotropic agents on energy coupling in everted membrane vesicles from aerobic and anaerobic cultures.Biochim Biophys Acta. 1977 Feb 7;459(2):225-40. doi: 10.1016/0005-2728(77)90024-x. Biochim Biophys Acta. 1977. PMID: 138439
-
The role of the carbodiimide-reactive component of the adenosine-5'-triphosphatase complex in the proton permeability of Escherichia coli membrane vesicles.Biochemistry. 1976 Jun 29;15(13):2741-6. doi: 10.1021/bi00658a005. Biochemistry. 1976. PMID: 132963
-
Energetics and molecular biology of active transport in bacterial membrane vesicles.J Supramol Struct. 1977;7(3-4):443-61. doi: 10.1002/jss.400070315. J Supramol Struct. 1977. PMID: 357844 Review.
-
[Membrane vesicles prepared by different procedures from Escherichia coli: orientation of membrane vesicles and coupling of energy to transport into membrane vesicles (author's transl)].Tanpakushitsu Kakusan Koso. 1975 Oct;20(12):1111-22. Tanpakushitsu Kakusan Koso. 1975. PMID: 172977 Review. Japanese. No abstract available.
Cited by
-
ATP-driven active transport in right-side-out bacterial membrane vesicles.Proc Natl Acad Sci U S A. 1981 Jun;78(6):3446-9. doi: 10.1073/pnas.78.6.3446. Proc Natl Acad Sci U S A. 1981. PMID: 6267592 Free PMC article.
-
Membrane potential and gentamicin uptake in Staphylococcus aureus.Proc Natl Acad Sci U S A. 1982 Nov;79(21):6693-7. doi: 10.1073/pnas.79.21.6693. Proc Natl Acad Sci U S A. 1982. PMID: 6959147 Free PMC article.
-
The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles.Proc Natl Acad Sci U S A. 1976 Jun;73(6):1892-6. doi: 10.1073/pnas.73.6.1892. Proc Natl Acad Sci U S A. 1976. PMID: 6961 Free PMC article.
-
Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation.Proc Natl Acad Sci U S A. 1977 Jun;74(6):2375-8. doi: 10.1073/pnas.74.6.2375. Proc Natl Acad Sci U S A. 1977. PMID: 18736 Free PMC article.
-
Membrane-associated DNase activity controlled by genes 46 and 47 of bacteriophage T4D and elevated DNase activity associated with the T4 das mutation.J Virol. 1981 Oct;40(1):65-77. doi: 10.1128/JVI.40.1.65-77.1981. J Virol. 1981. PMID: 7026800 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
