doi: 10.1097/00001756-199009000-00004.
Oligodendrocyte cell surface recognized by a novel monoclonal antibody specific to sulfatide
Affiliations
- PMID: 1717035
- DOI: 10.1097/00001756-199009000-00004
Item in Clipboard
Oligodendrocyte cell surface recognized by a novel monoclonal antibody specific to sulfatide
Neuroreport.
1990 Sep.
Abstract
A rat monoclonal antibody (OL-1) was obtained by in-vitro immunization of rat spleenocytes with paraformaldehyde-fixed primary cultured glial cells derived from newborn rat brain and subsequent fusion with a rat myeloma cell line. The antibody secreted by the hybridoma immunostains live rat and mouse oligodendrocytes in primary and secondary cultures. The antibody binds specifically to oligodendrocytes and myelin structures in-situ. Radioimmunolabelling assays with a number of purified glycolipids offer thin layer chromatography separation show that OL-1 antibody binds strongly to sulfatide and to a lesser extent to galactosyl diglyceride.
Similar articles
-
A sulfatide-reactive human monoclonal antibody obtained from a multiple sclerosis patient selectively binds to the surface of oligodendrocytes.J Neuroimmunol. 1995 Feb;56(2):191-200. doi: 10.1016/0165-5728(94)00150-m. J Neuroimmunol. 1995. PMID: 7860714
-
Generation and characterization of the binding epitope of a novel monoclonal antibody to sulfatide (sulfogalactosylceramide) OL-2: applications of antigen immunodetections in brain tissues and urinary samples.J Neuroimmunol. 2008 Jan;193(1-2):52-8. doi: 10.1016/j.jneuroim.2007.10.008. Epub 2007 Nov 19. J Neuroimmunol. 2008. PMID: 18023883
-
Monoclonal autoantibody SCH94.03, which promotes central nervous system remyelination, recognizes an antigen on the surface of oligodendrocytes.J Neurosci Res. 1996 Feb 1;43(3):273-281. doi: 10.1002/(SICI)1097-4547(19960201)43:3<273::AID-JNR2>3.0.CO;2-G. J Neurosci Res. 1996. PMID: 8714516
-
Novel oligodendrocyte transmembrane signaling systems. Investigations utilizing antibodies as ligands.Mol Neurobiol. 1993 Spring;7(1):1-22. doi: 10.1007/BF02780606. Mol Neurobiol. 1993. PMID: 8391270 Review.
-
Cell surface antigens in the nervous system.Scand J Immunol Suppl. 1982;9:201-22. doi: 10.1111/j.1365-3083.1982.tb03765.x. Scand J Immunol Suppl. 1982. PMID: 6763767 Review. No abstract available.
Cited by
-
Phenotype of arylsulfatase A-deficient mice: relationship to human metachromatic leukodystrophy.Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14821-6. doi: 10.1073/pnas.93.25.14821. Proc Natl Acad Sci U S A. 1996. PMID: 8962139 Free PMC article.
-
Expression of thyroid hormone receptor isoforms in the oligodendrocyte lineage.Neurochem Res. 2004 May;29(5):903-22. doi: 10.1023/b:nere.0000021235.83952.9a. Neurochem Res. 2004. PMID: 15139289 Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources