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. 2007 Jan;56(Pt 1):52-55.
doi: 10.1099/jmm.0.46909-0.

Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum beta-lactamases

Christopher I Birkett  1 , Hugo A Ludlam  1 , Neil Woodford  2 , Derek F J Brown  1 , Nicholas M Brown  1 , Mark T M Roberts  1 , Nicola Milner  1 , Martin D Curran  1
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Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum beta-lactamases

Christopher I Birkett et al. J Med Microbiol. 2007 Jan.

Abstract

The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla(CTX-M) genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on beta-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum beta-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla(CTX-M) genes in 21 of 28 ESBL-producing isolates.

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