Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2006 Dec;25(10):1227-30.
doi: 10.1097/01.ico.0000240083.31432.70.

Detection of Streptococcus species by polymerase chain reaction in infectious crystalline keratopathy

Yasuhiro Osakabe  1 , Chiemi Yaguchi, Takashi Miyai, Kazunori Miyata, Shoichiro Mineo, Masaki Nakamura, Shiro Amano
Affiliations
Case Reports

Detection of Streptococcus species by polymerase chain reaction in infectious crystalline keratopathy

Yasuhiro Osakabe et al. Cornea. 2006 Dec.

Abstract

Purpose: Investigation of a positive polymerase chain reaction (PCR) for Streptococcus species from an excised cornea of a patient with infectious crystalline keratopathy.

Methods: Samples microdissected from corneal intrastromal aggregates present in infectious crystalline keratopathy were subjected to PCR for 3 primer pairs respectively specific for Streptococcus, Staphylococcus, and several kinds of fungi.

Results: The PCR detected DNA from Streptococcus but not from Staphylococcus or fungi. Direct sequencing of the PCR products showed that the PCR products had the highest identity (99.8%) with Streptococcus mitis and Streptococcus gordoni, indicating that these species were the most probable causes of infectious crystalline keratopathy in this patient.

Conclusion: Our findings suggest that PCR provides a good adjunct technique in the diagnosis of corneal infection.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources