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. 1991 Aug 15;104(2):169-76.
doi: 10.1016/0378-1119(91)90247-9.

Sequence of EndoA gene encoding mouse cytokeratin and its methylation state in the CpG-rich region

Y Tamai  1 , Y Takemoto, M Matsumoto, T Morita, A Matsushiro, M Nozaki
Affiliations

Sequence of EndoA gene encoding mouse cytokeratin and its methylation state in the CpG-rich region

Y Tamai et al. Gene. .

Abstract

A genomic clone obtained from mouse liver DNA using a mouse cytokeratin EndoA cDNA probe revealed the complete sequence of the EndoA gene. The gene is divided into nine exons and the exon-intron pattern has been conserved compared to that of other type-II cytokeratin-encoding genes. The 5' upstream, 3' downstream and first and third introns contain potential regulatory sequences, including polyoma virus enhancer motifs (PEA1 and PEA3) and AP-1 elements. The 5' regions upstream of the EndoA, EndoB and Ck8 genes contain homologous sequences surrounding the TATA boxes. In addition, a CpG dinucleotide cluster region was located around the first exon. This CpG cluster region was found to be hypomethylated in endodermal PYS-2 cells, retinoic acid-treated F9 cells, and F9 embryonal carcinoma cells, but hypermethylated in BALB/C 3T3 fibroblast cells that do not express EndoA. These findings may provide a clue to understanding the molecular mechanisms of EndoA gene expression.

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